Biosynthesis of alkyl-ether containing lipid from dihydroxyacetone phosphate
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منابع مشابه
Biosynthesis of alkyl-ether containing lipid from dihydroxyacetone phosphate.
A lipid, stable to alkaline methanolysis, is formed when liver mitochondria or brain microsomes are incubated with dihydroxyacetone phosphate, long chain alcohol, ATP, CoA, NaF and Mg*. The lipid has been identified as alkyl dihydroxyacetone phosphate. This lipid is reduced by NADPH to 1-alkyl glycerol3-phosphate and is further acylated to form 1-alkyl 2-acyl glycerol-3-phosphate. The ether-con...
متن کاملAcyl dihydroxyacetone phosphate: precursor of alkyl ethers.
1-0-Alkyl dihydroxyacetone phosphate is biosynthesised in guinea pig liver mitochondria and in rat brain microsomes by direct reaction of a long chain alcohol and acyl dihydroxyacetone phosphate. ATP and Mg* are stimulatory but coenzyme A is not necessary for the reaction. 1-0-Alkyl dihydroxyacetone phosphate (alkyl DHAP)l was shown to be biosynthesized from long chain alcohol and DHAP with ATP...
متن کاملLoss of hydrogen from dihydroxyacetone phosphate during glyceryl ether synthesis.
In order to obtain information on the mechanism of ether bond formation in the synthesis of 0-alkyl lipids, a study was carried out to determine if there is a loss of hydrogen from carbon 3 of dihydroxyacetone phosphate (DHAP) (nonphosphorus-linked carbon) in the course of its incorporation into glyceryl ethers. Accordingly, a mixture of [1,3-3H2] DHAP and [I ,3-WJDHAP was prepared. In a micros...
متن کاملExclusive localization in peroxisomes of dihydroxyacetone phosphate acyltransferase and alkyl-dihydroxyacetone phosphate synthase in rat liver.
Dihydroxyacetone phosphate acyl transferase (DHAP-AT), alkyl dihydroxyacetone phosphate synthase (alkyl-DHAP-synthase), and glycerol-3-phosphate acyltransferase (GPAT) activities were investigated under optimal assay conditions using highly purified organelle preparations. The data presented clearly indicate that GPAT activity was mainly localized in mitochondria and microsomes, whereas DHAP-AT...
متن کاملAssay and properties of the enzyme catalyzing the biosynthesis of 1-O-alkyl dihydroxyacetone 3-phosphate.
The properties of the enzyme alkyl dihydroxyacetone phosphate synthase (1-O-acyl dihydroxyacetone 3-phosphate + ROH 1-0-alkyl dihydroxyacetone 3-phosphate + fatty acid) in Ehrlich ascites tumor cell microsomes were studied utilizing a new assay procedure. The assay is based on the difference of partition coefficient between alkyl dihydroxyacetone-P and hexadecanol in an alkaline two-phase CHC&-...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1969
ISSN: 0006-291X
DOI: 10.1016/0006-291x(69)90941-3